Sattlegger Lab

 Selected publications                                                               

* denotes corresponding author

Experimentally based structural model of Yih1 provides insight into its function in controlling the key translational regulator Gcn2.  Elena Harjes, Geoffrey B Jameson, Yi‐Hsuan Tu, Natalie Burr, Trevor S Loo, Alexander K Goroncy, Patrick JB Edwards, Stefan Harjes, Ben Munro, Christoph Göbl, Evelyn Sattlegger, Gillian E Norris.  FEBS letters, in press

Domain II of the translation elongation factor eEF1A is required for Gcn2 kinase inhibition.  (2020) Ramesh R & Sattlegger E.  FEBS Lett. 2020 594:2266-81. Online ahead of print.  Publication highlighted on the Journal?s website and promoted by the Journal through twitter. 

Damaging de novo missense variants in EEF1A2 lead to a developmental and degenerative epileptic-dyskinetic encephalopathy. (2020) Carvill GL, Padilla-Lopez S, Helbig KL, Myers C, Guida BS, Sebe J, Huether R, Kruer TN, Oktay SU, Ramsey K, Narayanan V, Feyma T, Tang S, Stickney H, Bandiwad AA, Sisneros JA, Rohena LO, Hollingsworth G, Jones C, Gill D, Depienne C, Nava C, Lin AE, Jansen FE, Vissers L, Willemsen MH, Kleefstra T, Sadleir LG, Sattlegger E, Sheffer I, Raible D,  Mefford H,* Kruer MC* Human Mutation 41:1263-1279.  Editor's Choice paper, considered particularly noteworthy by journal?s editorial board, will be available, at
https://onlinelibrary.wiley.com/doi/toc/10.1002/(ISSN)1098-1004.HUMU-Editors-Choice

Yeast as a model to understand actin-mediated cellular functions in mammals - illustrated with four actin cytoskeleton proteins. (2020) Akram Z, Ahmed I, Mack H, Kaur R, Silva R, Castilho B, Friant S, Sattlegger E, Munn A. invited review, Cells 9(3):672

Persistent contamination of Salmonella, Campylobacter, Escherichia coli, and Staphylococcus aureus at a broiler farm in New Zealand.  (2020) Castaneda-Gulla K, Sattlegger E, Mutukumira AN.  Can J Microbiol. 2020 66(3):171-185. Selected as 'Editors' Choice' paper. This initiative of Canadian Science Publishing and the Journal is a means of highlighting articles of particularly high caliber and topical importance.  https://www.nrcresearchpress.com/journal/cjm

Imprinted and ancient Gene: a potential mediator of cancer cell survival during tryptophan deprivation. (2018) Tomek P, Gore SK, Potts CL, Print CG, BLack MA, Hallemayr A, Killan M, Sattlegger E, Ching LM. Cell Communication and Signalling.16(1):88 https://rdcu.be/bbTL2

A Rapid Extraction Method for mammalian cell cultures, suitable for quantitative immunoblotting analysis of proteins, including phosphorylated GCN2 and eIF2alpha. (2017) Silva RC, Castilho BA, Sattlegger E*. MethodsX. 5:75-82 https://doi.org/10.1016/j.mex.2017.10.008

Cost-effective and rapid lysis of Saccharomyces cerevisiae cells for quantitative western blot analysis of proteins, including phosphorylated eIF2alpha.  (2017)  Lee SJ, Ramesh R, de Boor V, Gebler JM, Silva RC, Sattlegger E*.  Yeast. 34(9):371-382

Recognition of a structural domain (RWDBD) in Gcn1 proteins that interacts with the RWD domain containing proteins.  (2017) Rakesh R, Krishnan R, Sattlegger E*, Srinivasan N*.  Biology Direct 2017 12:12, DOI:10.1186/s13062-017-0184-3

Perturbations in actin dynamics reconfigure protein complexes that modulate GCN2 activity and promote an eIF2 response.  (2016) Silva RC, Sattlegger E, Castilho BA.  J. Cell Sci. 29(4):4521-4533 selected for inclusion in the 'In This Issue' section of JCS, http://jcs.biologists.org/content/129/24/e2404, demonstrating international recognition of our research finding. featured in Journal issue http://jcs.biologists.org/content/129/24/e2404

Re-using plastic cassettes for vertical gel electrophoresis. (2016) Ramesh R, Gebler JM, de Boor V, Sattlegger E.* Nature Protocol Exchange. doi: 10.1038/protex.2016.041

The Gcn2 Regulator Yih1 Interacts with the Cyclin Dependent Kinase Cdc28 and Promotes Cell Cycle Progression through G2/M in Budding Yeast. (2015) Silva RC, Dautel M, Di Genova BM, Amberg DC, Castilho BA,* Sattlegger E*. PLoS One. 10(7):e0131070.  

Gcn1 contacts the small ribosomal protein Rps10, which is required for full activation of the protein kinase Gcn2. (2015) Lee SJ, Swanson MJ, and SattleggerE,  Biochem J. 466:547-59

Yeast studies reveal moonlighting functions of the ancient actin cytoskeleton. (2014) Sattlegger E, Chernova TA, Gogoi NM, Pillai IV, Chernoff YO, and Munn* AL. peer-reviewed review. IUBMB Life 66:538-45

Keeping the eIF2 alpha kinase Gcn2 in check. (2014) Castilho BA, Shanmugam R, Silva RC, Ramesh R, Himme BM, Sattlegger* E. invited peer-reviewed review.  BBA Molecular Cell Research. 1843:1948-68. doi:10.1016/j.bbamcr.2014.04.006
Free access until 7/8/2014: http://authors.elsevier.com/a/1PD9Nc5IDEGrS

Evolutionarily conserved IMPACT impairs various stress responses that require GCN1 for activating the eIF2 kinase GCN2.  (2014)  Cambiaghi TD, Pereira CM, Shanmugam R, Bolech M, Wek RC, Sattlegger E, Castilho* BA. Biochem. Biophys. Res. Commun. 443:592-7

Semi-quantitative Colony Immunoassay for determining and optimizing protein expression inSaccharomyces cerevisiae and Escherichia coli.  (2014) Cridge AG, Visweswaraiah J, Ramesh R, Sattlegger* E. Anal. Biochem. 447:82-9

Transcriptional defect of an inherited NKX2-5 haplotype comprising a SNP, a nonsynonymous and a synonymous mutation, associated with human congenital heart disease. (2013)  Reamon-Buettner SM, Sattlegger E,  Ciribilli Y, Inga A, Wessel A, and Borlak* J.   PLoS ONE 8(12) e83295. DOI: 10.1371/journal.pone.0083295

Simultaneous semi-dry electrophoretic transfer of a wide range of differently sized proteins for immunoblotting. (2013)  Wiedemann M, Lee SJ, Silva RC, Visweswaraiah J, Soppert J, & Sattlegger* E. Nature Protocol Exchange. doi: 10.1038/protex.2013.095 

Overexpression of Eukaryotic Translation Elongation Factor 3 (eEF3) impairs Gcn2 activation. (2012) Visweswaraiah J, Lee SJ, Hinnebusch AG, and Sattlegger* E. J. Biol. Chem. 287: 37757-37768.  doi:10.1074/jbc.M112.368266 

Evidence that Yih1 resides in a complex with ribosomes.  (2012) Waller T, Lee SJ, and Sattlegger E. The FEBS J. 279(10):1761-76

Evidence that eukaryotic translation elongation factor 1A (eEF1A) binds the Gcn2 C-terminus and inhibits Gcn2 activation. ** (2011) Visweswaraiah J, Lageix S, Castilho BA, Izotova L, Kinzy TG, Hinnebusch AG, and Sattlegger* E. J. Biol. Chem. 286(42):36568-36579
** selected as 'Paper of the Week', in the week of 21 October 2011, (selected by JBC's Associate Editors and Editorial Board Members as representing (at that time) the top 1% of papers reviewed in terms of significance and overall importance).see http://www.jbc.org/potw 
and http://www.jbc.org/content/286/42/e99968.short 

Gcn1 and actin binding to Yih1: Implications for activation of the eIF2 kinase Gcn2.  (2011)  Sattlegger* E (*correponding author), Barbosa JARG, Moraes MCS, Martins RM, Hinnebusch AG, Castilho BA. J. Biol. Chem. 286(12):10341-55

Generating highly concentrated yeast whole cell extract using low-cost equipment. (2011) Visweswaraiah J, Dautel M, & Sattlegger* E. Nature Protocol Exchange. doi:10.1038/protex.2011.212

Saccharomyces cerevisiae Rbg1 protein and its binding partner Gir2 interact on Polyribosomes with Gcn1.  (2009) Wout PK, Sattlegger E, Sullivan SM, Maddock* JR.  Eukaryot Cell. 8(7):1061-71.

Yeast Studies Reveal New Roles for an Ancient Skeleton.  (2009) 
Munn* A & Sattlegger E.  Australian Biochemist.  40(8):8,9-13. review 

Sattlegger moved to New Zealand, applied for funding, and established own research group, therefore leading to a 'publication gap'.

Interplay between GCN2 and GCN4 expression, translation elongation factor 1 mutations and translational fidelity in yeast.  (2005) Magazinnik T, Anand M, Sattlegger E, Hinnebusch AG, Kinzy* TG.  Nucleic Acids Res. 33(14):4584-92.

IMPACT, a protein preferentially expressed in the mouse brain, binds GCN1 and inhibits GCN2 activation.  (2005) Pereira CM, Sattlegger E, Jiang HY, Longo BM, Jaqueta CB, Hinnebusch AG, Wek RC, Mello LE, Castilho* BA.  J Biol Chem. 280(31):28316-23.

Polyribosome binding by GCN1 is required for full activation of eukaryotic translation initiation factor 2alpha kinase GCN2 during amino acid starvation.* Sattlegger E, Hinnebusch* AG.  J Biol Chem. 280(16):16514-21.
* listed in Faculty of 1000 Biology, see http://f1000.com/signin?target=1024423 

YIH1 is an actin-binding protein that inhibits protein kinase GCN2 and impairs general amino acid control when overexpressed.  (2004) Sattlegger E, Swanson MJ, Ashcraft EA, Jennings JL, Fekete RA, Link AJ, Hinnebusch* AG.  J Biol Chem. 279(29):29952-62.

Biophysical characterization of Gir2, a highly acidic protein of Saccharomyces cerevisiae with anomalous electrophoretic behavior.  (2004) Alves VS, Pimenta DC, Sattlegger E, Castilho* BA.  Biochem Biophys Res Commun. 314(1):229-34.

Separate domains in GCN1 for binding protein kinase GCN2 and ribosomes are required for GCN2 activation in amino acid-starved cells.  (2000) Sattlegger E, Hinnebusch* AG.  EMBO J. 19(23):6622-33.

The WD protein Cpc2p is required for repression of Gcn4 protein activity in yeast in the absence of amino-acid starvation.  (1999) Hoffmann B, Mösch HU, Sattlegger E, Barthelmess IB, Hinnebusch A, Braus* GH.  Mol Microbiol. 31(3):807-22.

cpc-3, the Neurospora crassa homologue of yeast GCN2, encodes a polypeptide with juxtaposed eIF2alpha kinase and histidyl-tRNA synthetase-related domains required for general amino acid control.  (1998) Sattlegger* E, Hinnebusch AG, Barthelmess IB.  J Biol Chem. 273(32):20404-16.

The cpc-2 gene of Neurospora crassa encodes a protein entirely composed of WD-repeat segments that is involved in general amino acid control and female fertility.  (1995) Müller F, Krüger D, Sattlegger E, Hoffmann B, Ballario P, Kanaan M, Barthelmess* IB.  Mol Gen Genet. 248(2):162-73.

 Artistic cover illustration