Sattlegger Lab

This manuscript reports on a more sensitive and reliable Western detection of phospho-Gcn2, Gcn2, eIF2 and phospho-eIF2 in mammalian cells.  Other proteins can be detected with this method as well, of course.  The procedure is very fast, and also does not require expensive protease inhibitors.  see here:  http://www.sciencedirect.com/science/article/pii/S2215016117300456